![]() Total RNA was extracted from each population of microdissected cells using an Arcturus PicoPure RNA Isolation Kit (Cat# 12204-01).įor samples (ch1), 100 pg to 5 ng of total RNA was subjected to two rounds of T7 linear amplification using the Arcturus® RiboAmp® HS PLUS Kit (Catalog number: KIT0525) and labeled with C圓 dye (Arcturus® C圓 Turbo Labeling™ Kit Catalog number: KIT0609) according to the manufacturer’s protocol. All microdissections were performed within three hours of tissue staining. Normal and tumor epithelium and stroma were separately microdissected from tissue samples using a PixCell IIe LCM system (Arcturus). ![]() Tissue compartments isolated from OCT-embedded frozen human tissue samples by laser capture microscopy following HistoGene staining and pathologist's evaluation. Laser capture microdissected tissue, tumor epitheliumīreast tissue compartment: Tumor epithelium GEO help: Mouse over screen elements for information.
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